ABSTRACT
The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP), reverse passive hemogglutination (RPHA) and by micro-enzyme-linked-immunosorbent assay (ELISA) technique. Among the patients, HBsAg was detected in 12 cases (23%) by CIEP, in 18 cases (34%) by RPHA and in 23 patients (45%) by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7%) by CIEP, in 8 samples (3.5%) by RPHA and in 32 samples (13.5%) by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.
Subject(s)
Acute Disease , Adolescent , Adult , Female , Hepatitis B Surface Antigens/analysis , Hepatitis, Viral, Human/blood , Humans , Male , Middle AgedABSTRACT
During the ten year study i.e. 1978 to 1987, out of 53,251 cases of suspected enteric fever, S.typhi was isolated from 4005 cases (7%) and S.para typhi 'A' from 1108 cases (2.5%). A total of 52 strains of S. typhi and 4 strains of S.para typhi 'A' were found to be resistant to chloramphenicol. Minimal inhibitory concentration was greater than 256 micrograms/ml of all the strains of S.typhi and S.para typhi 'A'.
Subject(s)
Chloramphenicol/pharmacology , Chloramphenicol Resistance , Humans , India , Salmonella paratyphi A/drug effects , Salmonella typhi/drug effects , Typhoid Fever/drug therapyABSTRACT
The serum levels of IgA, IgG and IgM were determined by single radial immuno-diffusion technique in 104 male patients of acute viral hepatitis. HBsAg was detected by counterimmuno-electrophoresis (CIEP) and 51% were HBsAg + ve. IgA, IgG and IgM levels were found to be higher than normal levels. Mean IgA and IgG levels were same in HBsAg + ve and HBsAg--ve patients. Mean IgM levels were however, higher in HBsAg--ve patients.